Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria

Garba Ibrahim; Umar Asiya Imam; Ganau Ahmed Mohammed; Raji Mudasiru Iyanda Omowale; Fana Sani Abdullahi; Shinkafi Sa’adatu Aliyu; Kazeem Ademola

doi:doi:10.11648/j.ajbls.20200804.11

Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria

Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, Shinkafi Sa’adatu Aliyu, Kazeem Ademola

Published in American Journal of Biomedical and Life Sciences (Volume 8, Issue 4)

Received: 18 March 2020 Accepted: 14 April 2020 Published: 9 June 2020

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Abstract

Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.

Published in	American Journal of Biomedical and Life Sciences (Volume 8, Issue 4)
DOI	10.11648/j.ajbls.20200804.11
Page(s)	64-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Comparison, Microscopic, Rapid Test, Malaria, Sokoto

References

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Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, et al. (2020). Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. American Journal of Biomedical and Life Sciences, 8(4), 64-68. https://doi.org/10.11648/j.ajbls.20200804.11

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ACS Style

Garba Ibrahim; Umar Asiya Imam; Ganau Ahmed Mohammed; Raji Mudasiru Iyanda Omowale; Fana Sani Abdullahi, et al. Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. Am. J. Biomed. Life Sci. 2020, 8(4), 64-68. doi: 10.11648/j.ajbls.20200804.11

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AMA Style

Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, et al. Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. Am J Biomed Life Sci. 2020;8(4):64-68. doi: 10.11648/j.ajbls.20200804.11

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@article{10.11648/j.ajbls.20200804.11,
  author = {Garba Ibrahim and Umar Asiya Imam and Ganau Ahmed Mohammed and Raji Mudasiru Iyanda Omowale and Fana Sani Abdullahi and Shinkafi Sa’adatu Aliyu and Kazeem Ademola},
  title = {Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {8},
  number = {4},
  pages = {64-68},
  doi = {10.11648/j.ajbls.20200804.11},
  url = {https://doi.org/10.11648/j.ajbls.20200804.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20200804.11},
  abstract = {Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.},
 year = {2020}
}

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TY  - JOUR
T1  - Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria
AU  - Garba Ibrahim
AU  - Umar Asiya Imam
AU  - Ganau Ahmed Mohammed
AU  - Raji Mudasiru Iyanda Omowale
AU  - Fana Sani Abdullahi
AU  - Shinkafi Sa’adatu Aliyu
AU  - Kazeem Ademola
Y1  - 2020/06/09
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbls.20200804.11
DO  - 10.11648/j.ajbls.20200804.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 64
EP  - 68
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20200804.11
AB  - Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.
VL  - 8
IS  - 4
ER  -

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Author Information

Garba Ibrahim

Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Umar Asiya Imam

Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Ganau Ahmed Mohammed

Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Raji Mudasiru Iyanda Omowale

Department of Pharmaceutics and Pharm, Microbiology, Usmanu Danfodiyo University, Sokoto, Nigeria
Fana Sani Abdullahi

Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
Shinkafi Sa’adatu Aliyu

Department of Biological Sciences, Federal University, Gusau, Nigeria
Kazeem Ademola

Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria

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APA Style

Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, et al. (2020). Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. American Journal of Biomedical and Life Sciences, 8(4), 64-68. https://doi.org/10.11648/j.ajbls.20200804.11

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ACS Style

Garba Ibrahim; Umar Asiya Imam; Ganau Ahmed Mohammed; Raji Mudasiru Iyanda Omowale; Fana Sani Abdullahi, et al. Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. Am. J. Biomed. Life Sci. 2020, 8(4), 64-68. doi: 10.11648/j.ajbls.20200804.11

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AMA Style

Garba Ibrahim, Umar Asiya Imam, Ganau Ahmed Mohammed, Raji Mudasiru Iyanda Omowale, Fana Sani Abdullahi, et al. Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria. Am J Biomed Life Sci. 2020;8(4):64-68. doi: 10.11648/j.ajbls.20200804.11

Copy | Download

@article{10.11648/j.ajbls.20200804.11,
  author = {Garba Ibrahim and Umar Asiya Imam and Ganau Ahmed Mohammed and Raji Mudasiru Iyanda Omowale and Fana Sani Abdullahi and Shinkafi Sa’adatu Aliyu and Kazeem Ademola},
  title = {Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {8},
  number = {4},
  pages = {64-68},
  doi = {10.11648/j.ajbls.20200804.11},
  url = {https://doi.org/10.11648/j.ajbls.20200804.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20200804.11},
  abstract = {Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Comparison Between Microscopic and Rapid Test Assay in the Detection of Malaria Parasite Infection in Patient Attending Some Hospitals in Sokoto Nigeria
AU  - Garba Ibrahim
AU  - Umar Asiya Imam
AU  - Ganau Ahmed Mohammed
AU  - Raji Mudasiru Iyanda Omowale
AU  - Fana Sani Abdullahi
AU  - Shinkafi Sa’adatu Aliyu
AU  - Kazeem Ademola
Y1  - 2020/06/09
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbls.20200804.11
DO  - 10.11648/j.ajbls.20200804.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 64
EP  - 68
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20200804.11
AB  - Malaria is a serious public health problem, this study was aimed at comparing Microscopy and Rapid Diagnostics test in malaria parasite detection in patients in some hospitals in Sokoto, Nigeria. Blood Samples were collected by venipuncture and dispensed into EDTA bottle. Thick blood films were made by adding few drops of anticoagulated blood on a clean glass slides and emulsifying to coin size using a spreader and allowed to air-dry and stained with 10% Geimsa stain for 10 minutes, allowed to air-dry and examined using ×100 objective to confirm the presence of malaria parasites. Of the 100 patients screened, 37% and 24% were positive for malaria using Carestart and SD-Bioline, while 53% were malaria positive by microscopy. Prevalence of malaria parasite by sex was 53.6% and 52.3% in females and males. Malaria prevalence by age was highest among patients aged 11-20, while the lowest prevalence was observed in patients aged 51-60 years. Based on occupation, the prevalence of malaria was highest among unemployed patients 58.5%, followed by 22.6% prevalence of malaria among business men and women. No significant difference was observed in malaria infection by sex, age and occupations P<0.05. The sensitivity and specificity of Carestart and SD-Bioline screening kits were 54.7% and 82.6%, 34.6 and 87.2%. The predictive positive value of Carestart was 78.4% while its negative predictive value was 61.3% in contrast to SD-Bioline with 75% and 54.7% as the positive predictive value and negative predictive value. Rapid diagnostic test are suitable alternatives to microscopy particularly in resources limited rural areas.
VL  - 8
IS  - 4
ER  -

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